Vitamin D Assays in Clinical Laboratory: Past, Present and Future Challenges

Published on : 30 March 2016
Location : Abu Dhabi

 

 

Dr. Shereen Atef, National Reference Laboratory Consultant Clinical Pathologist presented at the “5th International Conference on Vitamin D deficiency and Human Health” that took place at Jumeirah Etihad Towers, Abu Dhabi on March 24 - 25, 2016.

Dr. Shereen had the presentation on the first conference day – March 24th, from 14:15 to 14:40, on the topic of “Vitamin D Assay in Clinical Laboratory: Past, Present and Future Challenges”. Please find below the abstract of her lecture.

 

Vitamin D Assays in Clinical Laboratory: Past, Present and Future Challenges   

Over the last years, there has been a dramatic increase in requests for testing of 25-hydroxyvitamin D (25(OH) D). With the availability of many vitamin D assays, differences in the reported 25(OH)D values were observed in different assays. This could impact the classification of patients’ vitamin D status and affect the clinical management.

Serum concentration of 25(OH) D substantially varies depending on the type of the assay, which can be a binding or a chemical assay.

The binding assay group includes chemiluminescence immunoassays (CLIA), radioimmunoassy (RIA), and binding protein assay. The chemical assay includes high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Significant differences in the results of 25(OH)D were observed among the various assays. Standardization and harmonization of 25(OH)D measurements are therefore urgently needed. The widespread introduction of well-standardized assays in clinical laboratories is the challenge in the next years.